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Servicebio Inc rapid blocking solution
Rapid Blocking Solution, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rapid+blocking+solution/blocking+solution/pm42061695-76-4-7
Average 86 stars, based on 1 article reviews
rapid blocking solution - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Blocking Assay:

Article Title: Global immunostimulatory mechanisms of a high-branched glycogen from Glyptocidaris crenularis eggs in RAW264.7 macrophages via BONCAT-based nascent proteomics
Article Snippet: Marine polysaccharides have emerged as promising immunomodulators.. However, conventional analytical approaches have failed to elucidate their activation mechanisms from a global perspective.. In this study, we isolated a highly branched glycogen (GCEP2a) from eggs of the sea urchin Glyptocidaris crenularis.

Article Title: Effects of dietary glutamine supplementation on lipid metabolism, fatty acid composition, and taste properties in the longissimus thoracis muscle of feed-restricted yaks
Article Snippet: .. In the next step, the PVDF membrane was blocked with a rapid blocking solution (G2052, Servicebio Technology Co., Ltd., Wuhan, Hubei, China) for 5 min at room temperature. .. The primary antibody was incubated overnight at 4 °C with the membranes, followed by the secondary antibody for 1.5 h. Target proteins were detected using a highly sensitive enhanced chemiluminescence (ECL) reagent (724D281, Oriscience Biological Technology Co., Ltd., Chengdu, Sichuan, China) and visualized with a chemiluminescence imaging system (AGP2307Y05, eBlot Photoelectric Technology Co., Ltd., Shanghai, China).

Article Title: Ginkgolide B Alleviates LPS-Induced Inhibition of Osteogenic Differentiation in Human Periodontal Ligament Stem Cells by Suppressing the p-IκBα/NF-κB Pathway
Article Snippet: .. After transferring the target proteins onto the PVDF membrane, the membrane was blocked using rapid blocking solution (Servicebio, Wuhan, China) for 15 min at room temperature. .. After thorough washing with Tris-buffered saline containing 0.1% Tween 20 (TBST) (Beyotime, Shanghai, China), the samples were incubated overnight at 4 °C with the following primary antibodies: anti-ALP (1:1000; Proteintech, Hubei, China), anti-RUNX2 (1:1000; ImmunoWay, Plano, TX, USA), anti-COL-1 (1:1000; Proteintech, Hubei, China) and anti-GAPDH (1:5000; Proteintech, Hubei, China) as loading control.

Article Title: 5-HEPE Ameliorates Aging of Duck Ovarian Granulosa Cells by Targeting FOXM1 and Suppressing Oxidative Stress
Article Snippet: .. After transfer, the membranes were treated with a rapid blocking solution (Servicebio, Wuhan, China) for 5 min at room temperature. .. Blots were then probed with primary antibodies against FOXM1 (ab245309, Abcam (Cambridge, UK)), GPX4 (3038-1-AP, Proteintech (Rosemont, IL, USA)) and β-actin (10536-1-AP, Proteintech) at 4 °C.

Article Title: Curculigo orchioides Polysaccharide Promotes the Growth and Development of Wenchang Chickens via the PI3K/Akt/mTOR Signaling Pathway
Article Snippet: .. Membranes were blocked with rapid blocking solution (G2052-500ML, Servicebio, Wuhan, China) for 1 h before incubation with specific primary antibodies. ..

Article Title: Effects of dietary glutamine supplementation on lipid metabolism, fatty acid composition, and taste properties in the longissimus thoracis muscle of feed-restricted yaks
Article Snippet: .. In the 211 Jo urn al Pr e-p roo f next step, the PVDF membrane was blocked with a rapid blocking solution (G2052, 212 Servicebio Technology Co., Ltd., Wuhan, Hubei, China) for 5 min at room temperature. .. 213 The primary antibody was incubated overnight at 4 °C with the membranes, followed 214 by the secondary antibody for 1.5 h. Target proteins were detected using a highly 215 sensitive enhanced chemiluminescence (ECL) reagent (724D281, Oriscience 216 Biological Technology Co., Ltd., Chengdu, Sichuan, China) and visualized with a 217 chemiluminescence imaging system (AGP2307Y05, eBlot Photoelectric Technology 218 Co., Ltd., Shanghai, China).

Article Title: Acetyltransferase p300 Promotes NNK-Induced Colorectal Cancer Progression by Mediating ANKHD1 Expression via H3K27ac Modification.
Article Snippet: Cigarette smoking significantly accelerates the initiation and progression of colorectal cancer (CRC), although the precise molecular mechanisms remain incompletely elucidated.. Among tobacco-derived carcinogens, 4(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a key one, which has been demonstrated to enhance the malignant progression of CRC.. CUT&RUN-seq and mRNA-seq analyses, along with subsequent validation experiments, reveal that NNK upregulates the expression of the acetyltransferase p300.

Incubation:

Article Title: Global immunostimulatory mechanisms of a high-branched glycogen from Glyptocidaris crenularis eggs in RAW264.7 macrophages via BONCAT-based nascent proteomics
Article Snippet: Marine polysaccharides have emerged as promising immunomodulators.. However, conventional analytical approaches have failed to elucidate their activation mechanisms from a global perspective.. In this study, we isolated a highly branched glycogen (GCEP2a) from eggs of the sea urchin Glyptocidaris crenularis.

Article Title: Curculigo orchioides Polysaccharide Promotes the Growth and Development of Wenchang Chickens via the PI3K/Akt/mTOR Signaling Pathway
Article Snippet: .. Membranes were blocked with rapid blocking solution (G2052-500ML, Servicebio, Wuhan, China) for 1 h before incubation with specific primary antibodies. ..

Article Title: Acetyltransferase p300 Promotes NNK-Induced Colorectal Cancer Progression by Mediating ANKHD1 Expression via H3K27ac Modification.
Article Snippet: Cigarette smoking significantly accelerates the initiation and progression of colorectal cancer (CRC), although the precise molecular mechanisms remain incompletely elucidated.. Among tobacco-derived carcinogens, 4(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a key one, which has been demonstrated to enhance the malignant progression of CRC.. CUT&RUN-seq and mRNA-seq analyses, along with subsequent validation experiments, reveal that NNK upregulates the expression of the acetyltransferase p300.

Membrane:

Article Title: Effects of dietary glutamine supplementation on lipid metabolism, fatty acid composition, and taste properties in the longissimus thoracis muscle of feed-restricted yaks
Article Snippet: .. In the next step, the PVDF membrane was blocked with a rapid blocking solution (G2052, Servicebio Technology Co., Ltd., Wuhan, Hubei, China) for 5 min at room temperature. .. The primary antibody was incubated overnight at 4 °C with the membranes, followed by the secondary antibody for 1.5 h. Target proteins were detected using a highly sensitive enhanced chemiluminescence (ECL) reagent (724D281, Oriscience Biological Technology Co., Ltd., Chengdu, Sichuan, China) and visualized with a chemiluminescence imaging system (AGP2307Y05, eBlot Photoelectric Technology Co., Ltd., Shanghai, China).

Article Title: Ginkgolide B Alleviates LPS-Induced Inhibition of Osteogenic Differentiation in Human Periodontal Ligament Stem Cells by Suppressing the p-IκBα/NF-κB Pathway
Article Snippet: .. After transferring the target proteins onto the PVDF membrane, the membrane was blocked using rapid blocking solution (Servicebio, Wuhan, China) for 15 min at room temperature. .. After thorough washing with Tris-buffered saline containing 0.1% Tween 20 (TBST) (Beyotime, Shanghai, China), the samples were incubated overnight at 4 °C with the following primary antibodies: anti-ALP (1:1000; Proteintech, Hubei, China), anti-RUNX2 (1:1000; ImmunoWay, Plano, TX, USA), anti-COL-1 (1:1000; Proteintech, Hubei, China) and anti-GAPDH (1:5000; Proteintech, Hubei, China) as loading control.

Article Title: Effects of dietary glutamine supplementation on lipid metabolism, fatty acid composition, and taste properties in the longissimus thoracis muscle of feed-restricted yaks
Article Snippet: .. In the 211 Jo urn al Pr e-p roo f next step, the PVDF membrane was blocked with a rapid blocking solution (G2052, 212 Servicebio Technology Co., Ltd., Wuhan, Hubei, China) for 5 min at room temperature. .. 213 The primary antibody was incubated overnight at 4 °C with the membranes, followed 214 by the secondary antibody for 1.5 h. Target proteins were detected using a highly 215 sensitive enhanced chemiluminescence (ECL) reagent (724D281, Oriscience 216 Biological Technology Co., Ltd., Chengdu, Sichuan, China) and visualized with a 217 chemiluminescence imaging system (AGP2307Y05, eBlot Photoelectric Technology 218 Co., Ltd., Shanghai, China).

Transferring:

Article Title: Ginkgolide B Alleviates LPS-Induced Inhibition of Osteogenic Differentiation in Human Periodontal Ligament Stem Cells by Suppressing the p-IκBα/NF-κB Pathway
Article Snippet: .. After transferring the target proteins onto the PVDF membrane, the membrane was blocked using rapid blocking solution (Servicebio, Wuhan, China) for 15 min at room temperature. .. After thorough washing with Tris-buffered saline containing 0.1% Tween 20 (TBST) (Beyotime, Shanghai, China), the samples were incubated overnight at 4 °C with the following primary antibodies: anti-ALP (1:1000; Proteintech, Hubei, China), anti-RUNX2 (1:1000; ImmunoWay, Plano, TX, USA), anti-COL-1 (1:1000; Proteintech, Hubei, China) and anti-GAPDH (1:5000; Proteintech, Hubei, China) as loading control.

other:

Article Title: YTHDF1-mediated RNA m1A methylation promotes malignant progression of hepatocellular carcinoma via regulating LRP5/Wnt-β-catenin axis.
Article Snippet: After boiling the mixture of proteins and loading the buffer (LT101, Epizyme) at 100 ◦C (5 min), equal proteins were separated via electrophoresis within sodium dodecyl sulfate-polyacrylamide gel and subsequently electro-transferred onto the polyvinylidene fluoride (PVDF) membranes.

Control:

Article Title: Acetyltransferase p300 Promotes NNK-Induced Colorectal Cancer Progression by Mediating ANKHD1 Expression via H3K27ac Modification.
Article Snippet: Cigarette smoking significantly accelerates the initiation and progression of colorectal cancer (CRC), although the precise molecular mechanisms remain incompletely elucidated.. Among tobacco-derived carcinogens, 4(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a key one, which has been demonstrated to enhance the malignant progression of CRC.. CUT&RUN-seq and mRNA-seq analyses, along with subsequent validation experiments, reveal that NNK upregulates the expression of the acetyltransferase p300.



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